Abscisic acid (ABA)- and methyl jasmonate (MeJA)-induced stomatal closure are accompanied by cytosolic alkalization
نویسندگان
چکیده
stomatal closure are accompanied by cytosolic alkalization in guard cells. However, it remains to be clarifi ed how the alkalization functions in not only ABA signaling but also MeJA. We investigated cytosolic alkalization in guard cells during ABA-, MeJAand Ca 2 + -induced stomatal closure of wild type, abi1-1 , abi2-1 , ost1-2 and coi1 using a pHsensitive fl uorescent dye, BCECF-AM. ABA induced cytosolic alkalization in guard cells of wild-type and coi1 but not in ost1-2 guard cells whereas MeJA elicited cytosolic alkalization in wild-type and ost1-2 guard cells but not in coi1 . Neither ABA nor MeJA induced cytosolic alkalization in abi1-1 and abi2-1 guard cells. Exogenous Ca 2 + induced stomatal closure accompanied by cytosolic alkalization in guard cells of wild-type, abi1-1 , abi2-1 , ost1-2 and coi1 plants. An agent to acidify cytosol, butyrate, suppressed Ca 2 + -induced cytosolic alkalization and ABA-, MeJAand Ca 2 + -induced cytosolic Ca 2 + oscillation in wild-type guard cells to prevent stomatal closure. These results indicate that cytosolic alkalization and cytosolic Ca 2 + oscillation coordinately function in ABA and MeJA signaling in Arabidopsis guard cells.
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